Part:BBa_K2804002

Bone morphogenetic protein 2 (BMP2)

The human bone morphogenetic protein 2 is a bone-growth regulatory factor well known to be a potent osteoinductive protein.

Characterization of BMP2's effects in cartilage regeneration (by KEYSTONE_A 2020)

BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro (Figure 3). Skeletal stem-cells (SSCs) isolated from the articular surface, periosteum and bone marrow all produced higher amounts of cartilage with addition of BMP2 + sVEGFR1 when compared with addition of PBS only (Figure 4). For in vivo experiments, cotransplantation with BMP2 + sVEGFR1 can also promote cartilage formation (Figure 5).

Reference

Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.

Figure 3. SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.

Figure 4. Alcian blue staining (for acidic glycans in cartilage) of SSC following chondrogenic differentiation with treatment of PBS or BMP2 + sVEGFR1

Figure 5. Cotransplantation with BMP2 + sVEGFR1 led to increased cartilage formation in vivo

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]